Summary: We performed single-nucleus RNA sequencing (snRNA-seq) of female midguts across five developmental stages, two genotypes (esg-GAl4> control, esg-GAl4>TetRNAi) spanning ISC specification: L3 larva, 0hr pupa (P0), 18hr pupa (P18), 48hr pupa (P48), and day-2 adult. In total, we obtained 10 samples with 121k high quality cells.
Data visualization and analysis: CellxGene
Raw and Processed data are available at NCBI/GEO (GSE327108).
Reference: Niccole Auld et al., Tet governs transcriptional programs required for intestinal stem cell development and homeostasis. submitted
contact: Hongjie.li@bcm.edu
Hongjie Li Lab 